Journal: PLoS ONE

Article Title: Bilateral Nerve Alterations in a Unilateral Experimental Neurotrophic Keratopathy Model: A Lateral Conjunctival Approach for Trigeminal Axotomy

doi: 10.1371/journal.pone.0070908

Figure Lengend Snippet: Representative histological photographs of corneal nerves stained with anti-βIII tubulin FITC-conjugated antibody. Normal subbasal (A) and stromal (E) nerve plexus. Subbasal nerve plexus completely disappeared (B; day 1, C; day 7, D; day 14) and stromal nerve definitely decreased from day1 (F; day 1, G; day 7, H; day 14) and after trigeminal axotomy. The nerve of the contralateral eye decreased from day 1 (J; day 1, K; day 7, L; day 14).

Article Snippet: Corneas were then stained with monoclonal NL637-conjugated anti-β-III tubulin antibody (anti-Neuron-specific β-III Tubulin-NL637, R&D systems Inc. Minneapolis, MN; dilution of 1∶100) at 4C degree overnight.

Techniques: Staining

Journal: PLoS ONE

Article Title: Bilateral Nerve Alterations in a Unilateral Experimental Neurotrophic Keratopathy Model: A Lateral Conjunctival Approach for Trigeminal Axotomy

doi: 10.1371/journal.pone.0070908

Figure Lengend Snippet: Representative histological photographs of corneal nerves stained with anti-βIII tubulin FITC-conjugated antibody. Normal subbasal (A) and stromal (E) nerve plexus.Subbasal nerve plexus completely disappeared (B; day 1, C; day 7, D; day 14) (A) and stromal nerve definitely decreased (B) from day 1 (F; day 1, G; day 7, H; day 14) after trigeminal axotomy. The nerve of the contralateral eye did not change (J; day 1, K; day 7, L; day 14).

Article Snippet: Corneas were then stained with monoclonal NL637-conjugated anti-β-III tubulin antibody (anti-Neuron-specific β-III Tubulin-NL637, R&D systems Inc. Minneapolis, MN; dilution of 1∶100) at 4C degree overnight.

Techniques: Staining

Journal: Journal of neurochemistry

Article Title: Embryonic stem cell-derived neuron models of Parkinson's disease exhibit delayed neuronal death.

doi: 10.1111/j.1471-4159.2006.03815.x

Figure Lengend Snippet: Fig. 2 (a) Expression of PARK gene products in Nurr1-ES (Control), aS WT-ES (aS WT), aS A30P-ES (aS A30P), and aS A53T-ES (aS A53T) cell lines at stage 1 or stage 5. Samples (10 lg) of the cell lysate or C57BL/6 mouse midbrain homogenates (Midbrain) were immunoblotted with anti-a-synuclein, anti-HA, anti-Parkin, anti-UCH- L1, anti-PINK1, anti-DJ-1, anti-synphilin-1, or anti-a-tubulin antibodies. (b) Expression levels of a-synuclein proteins in each of six inde- pendent clones for each cell line at stage 5. Samples (10 lg) of the cell lysates were immunoblotted with anti-a-synuclein antibodies. The degree of a-synuclein protein expression was quantitated by densito- metric analysis and data were represented as the x-fold of a-synuclein protein level in clone 1 of the Nurr1-ES cell line. Results are mean- s ± SEM for four independent experiments.

Article Snippet: 10-lg samples of the cell lysates were separated on polyacrylamide gels, transferred onto nitrocellulose membranes, and immunoblotted with anti-Nurr1 (1 : 1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-Myc (1 : 1000; Santa Cruz Biotechnology), anti-neuron-specific b-III tubulin (TuJ1; 1 : 1000; R & D Systems), anti-neural nuclei (NeuN; 1 : 1000; Chemicon, Temecula, CA, USA), anti-tyrosine hydroxylase (TH; 1 : 1000; Chemicon), anti-Parkin (1 : 1000; Cell Signaling Technology, Beverly, MA USA), anti-ubiquitin C-terminal hydrolase L1 (UCH-L1; 1 : 1000; Chemicon), anti-DJ-1 (1 : 1000; MBL, Nagoya, Japan), anti-PINK1 (1 : 1000; Oxford Biotechnology, Oxfordshire, UK), anti-synphilin-1 (1 : 1000; Nagano et al. 2003), anti-a-tubulin (1 : 1000; Santa Cruz Biotechnology), anti-a-synuclein (1 : 1000; BD Biosciences), or anti-HA (1 : 1000; Santa Cruz Biotechnology) antibodies.

Techniques: Expressing, Control, Clone Assay

Journal: Scientific Reports

Article Title: Intranasal delivery of nanomicelle curcumin promotes corneal epithelial wound healing in streptozotocin-induced diabetic mice

doi: 10.1038/srep29753

Figure Lengend Snippet: The effect of 7-day treatment with topical and/or intranasal nanomicelle curcumin in promoting diabetic corneal nerve regeneration after corneal epithelium abrasion was investigated. Corneas were harvested, flat-mounted, and immunostained with β-III tubulin antibody. ( A ) Images of central and peripheral corneal nerve were taken and combined as a whole flat-mounted corneal nerve. ( B ) Central and peripheral corneal nerve densities were calculated and are expressed as a percentage of that in the NC group (* P < 0.05 when compared with the DC group; ** P < 0.01, with the DC group; # P < 0.05, with the OT group; n = 3).

Article Snippet: Corneal nerve staining was performed, as described previously, with an anti-neuron-specific β-III tubulin antibody (NL1195R; R&D System Inc. Minneapolis, USA).

Techniques: